Purpose: In this lab experiment the mixtures of organic compounds are crystalized employ liquid chromatography. tender loving care separation takes curtilage on a thin layer of solid stationary phase scattering on a solid victuals as a liquid mobile phase, also only if called solvent, moves along the plate. In particular a Cartotenoid called Bixin is isolated from a mixture of Carotenoids put up in annatto seeds in this experiment. Although this technique is often use to separate uncolored mixtures of compounds in this exemplar colored compounds are used so that it is possible to come the separation as it occurs. Reagent submit: M.W(g)Molecular form Bixin394.5096C25H30O4 Methylbixin408.236C26H32O4 Norbixin380.216C24H28O4 All E-Geranylgeraniol288.194C20H32O Geranylgeraniol esters315.198C21H31O2 Phytoene541.4C40H64 Phytofluene542.896C40H60 Carotene536.88C40H56 Nurosprene538.864C40H58 force: Place 2.0 grams of small annatto seeds in a 50 mL beaker. To the beaker furnish a magnetic advance barr and 5 mL of 8% ethyl alcohol in methylene chloride tooth root. Allow the effect to stir for 5 to 10 minutes. Vacuum filter the resultant role by a movement fritted filter. Transfer the dark chromatic filter out to a clean 50 mL beaker. Transfer the filtered solids tail into the original beaker.
Repeat the extraction of the crushed annatto seed residual with 5 mL of the 8% Ethanol in methylene chloride declaration two more times. distributively time vacuum cleaner filter the orange solution through a course fritted funnel. Combine the annatto extracts into the same 50 mL beaker as the basic extract. After completing the TL! C analysis place a toiling chip in the annatto extract in the beaker and boil the solvent until only nigh 1 mL remains. Do not boil the dryness. The solution spatters and bumps if boiled to dryness. Remove the be solvent by momentary compressed air or nitrogen over... If you want to labour a full essay, golf club it on our website:
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